NFKB2,p100 Rekombinanter Antikörper

NFKB2,p100 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241126F1

Kat-Nr. : 84022-6-PBS

Synonyme

NFKB2, 241126F1, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1

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  WB analysis using 84022-6-RR (same clone as 84022-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84022-6-RR (NFKB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84022-6-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 84022-6-RR (same clone as 84022-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84022-6-RR (NFKB2,p100 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84022-6-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84022-6-RR (same clone as 84022-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NFKB2 antibody (84022-6-RR, Clone: 241126F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84022-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84022-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84022-6-RR against Human NFKB2,p100 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84022-6-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NFKB2,p100 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NFKB2,p100 fusion protein Ag0662
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC002844
• Gene symbol: NFKB2
• Gene ID (NCBI): 4791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.