NFKBIZ Polyklonaler Antikörper

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NFKBIZ Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14014-1-PBS

Synonyme

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  WB analysis of HeLa using 14014-1-AP (same clone as 14014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14014-1-AP (same clone as 14014-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14014-1-AP (same clone as 14014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14014-1-AP (same clone as 14014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14014-1-AP (same clone as 14014-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKBIZ antibody (14014-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14014-1-PBS bindet in WB, IF/ICC, Indirect ELISA NFKBIZ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFKBIZ fusion protein Ag5120
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa, 64 kDa
• GenBank-Zugangsnummer: BC060800
• Gene symbol: NFKBIZ
• Gene ID (NCBI): 64332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFKBIZ, also named as NF-kappa-B inhibitor zeta, is a 718 amino acid protein, which contains 7 ANK repeats and is expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. NFKBIZ exists as three isoforms and molecular weight of isoform three is a 64 kDa. The isoform one is 78 kDa protein and isoform two is a 68 kDa protein. NFKBIZ is involved in regulation of NF-kappa-B transcription factor complexes. It Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation.