NFS1 Polyklonaler Antikörper

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• KD/KO Validated

NFS1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 15370-1-AP

Synonyme

NIFS, IscS, HUSSY-08, HUSSY 08, EC:2.8.1.7

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  WB analysis of HepG2 using 15370-1-AP

  WB result of NFS1 antibody (15370-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells.

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  WB analysis of A549 using 15370-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15370-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15370-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15370-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15370-1-AP (NFS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15370-1-AP

  IP result of anti-NFS1 (IP:15370-1-AP, 4ug; Detection:15370-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human liver using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15370-1-AP (NFS1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15370-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15370-1-AP (NFS1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15370-1-AP

  Immunofluorescent analysis of HepG2 cells, using NFS1 antibody 15370-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 15370-1-AP

  Immunofluorescent analysis of HepG2 cells using 15370-1-AP (NFS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

15370-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NFS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFS1 fusion protein Ag3872
• Vollständiger Name: NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC018471
• Gene symbol: NFS1
• Gene ID (NCBI): 9054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
mouse	WB	J Nanobiotechnology; Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.; Authors - Li Xu
mouse	WB	FASEB J; PMID: 31530015; Authors - Rufeng Zhang
human	WB,IF	PLoS One; Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.; Authors - Ioana Ferecatu ; KD Validated
human	IHC	Discov Oncol; L-cysteine contributes to destructive activities of odontogenic cysts/tumor; Authors - Ji Li
rat	WB	Antioxidants (Basel); Downregulation of Iron-Sulfur Cluster Biogenesis May Contribute to Hyperglycemia-Mediated Diabetic Peripheral Neuropathy in Murine Models; Authors - Lin Wu