NFS1 Monoklonaler Antikörper

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• KD/KO Validated

NFS1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3A10A7

Kat-Nr. : 67021-1-Ig

Synonyme

3A10A7, Cysteine desulfurase, EC:2.8.1.7, HUSSY 08, HUSSY-08

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  WB analysis of HSC-T6 using 67021-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67021-1-Ig

  WB result of NFS1 antibody (67021-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells.

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  WB analysis of NIH/3T3 using 67021-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 67021-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67021-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67021-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67021-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67021-1-Ig bindet in WB, IHC, IF, ELISA NFS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NFS1 fusion protein Ag8017
• Vollständiger Name: NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC018471
• Gene symbol: NFS1
• Gene ID (NCBI): 9054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.

Publikationen

Species	Application	Title
human,rat	WB,IHC,IF	Free Radic Biol Med; GLS1-mediated glutamine metabolism mitigates oxidative stress-induced matrix degradation, ferroptosis, and senescence in nucleus pulposus cells by modulating Fe2+ homeostasis; Authors - Jiajun Wu
human	WB	Redox Biol; Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation; Authors - Jiayan Huang ; KD Validated