NFU1 Polyklonaler Antikörper

NFU1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25598-1-AP

Synonyme

CGI 33, HIRA interacting protein 5, HIRIP5, Nfu, NFU1, NifU, NIFUC

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  WB analysis of mouse cerebellum using 25598-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25598-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse cerebellum using 25598-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 25598-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 25598-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 25598-1-AP

  IP result of anti-NFU1 (IP:25598-1-AP, 4ug; Detection:25598-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IF Staining of HepG2 using 25598-1-AP

  Immunofluorescent analysis of HepG2 cells using 25598-1-AP (NFU1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Maushirngewebe, Maushodengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25598-1-AP bindet in WB, IF/ICC, IP, ELISA NFU1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFU1 fusion protein Ag22343
• Vollständiger Name: NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 254 aa, 28 kDa
• Beobachtetes Molekulargewicht: 23-28 kDa
• GenBank-Zugangsnummer: BC113692
• Gene symbol: NFU1
• Gene ID (NCBI): 27247
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFU1, also named as HIRA-interacting protein 5 or CGI-33, is a 254 amino acid protein, which belongs to the NifU family. NFU1 localizes in the Mitochondrion. NFU1 is widely expressed in adult tissues. NFU1, as an Iron-sulfur cluster scaffold protein, which can assemble clusters and deliver them to target proteins. It has an association with Multiple mitochondrial dysfunctions syndrome 1.

Publikationen

Species	Application	Title
human	WB	Science; Autoregulatory control of mitochondrial glutathione homeostasis; Authors - Yuyang Liu