NFU1 Polyklonaler Antikörper

NFU1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25598-1-PBS

Synonyme

---

• 

  WB analysis of mouse cerebellum using 25598-1-AP (same clone as 25598-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 25598-1-AP (same clone as 25598-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 25598-1-AP (same clone as 25598-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 25598-1-AP (same clone as 25598-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 25598-1-AP (same clone as 25598-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 25598-1-AP (same clone as 25598-1-PBS)

  IP result of anti-NFU1 (IP:25598-1-AP, 4ug; Detection:25598-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 25598-1-AP (same clone as 25598-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 25598-1-AP (NFU1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

25598-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA NFU1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFU1 fusion protein Ag22343
• Vollständiger Name: NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 254 aa, 28 kDa
• Beobachtetes Molekulargewicht: 23-28 kDa
• GenBank-Zugangsnummer: BC113692
• Gene symbol: NFU1
• Gene ID (NCBI): 27247
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFU1, also named as HIRA-interacting protein 5 or CGI-33, is a 254 amino acid protein, which belongs to the NifU family. NFU1 localizes in the Mitochondrion. NFU1 is widely expressed in adult tissues. NFU1, as an Iron-sulfur cluster scaffold protein, which can assemble clusters and deliver them to target proteins. It has an association with Multiple mitochondrial dysfunctions syndrome 1.