NGDN Polyklonaler Antikörper

NGDN Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16524-1-AP

Synonyme

LCP5, EIF4E-binding protein, EIF4E binding protein, Centromere accumulated nuclear protein 1, CANu1

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  WB analysis of human cerebellum using 16524-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16524-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human epididymis using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human epididymis using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16524-1-AP

  Immunofluorescent analysis of Hela cells, using NGDN antibody 16524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Cerebellum-Gewebe, Maushirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Nebenhodengewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16524-1-AP bindet in WB, IHC, IF/ICC, ELISA NGDN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NGDN fusion protein Ag9763
• Vollständiger Name: neuroguidin, EIF4E binding protein
• Berechnetes Molekulargewicht: 315 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC030817
• Gene symbol: NGDN
• Gene ID (NCBI): 25983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Nature; A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis.; Authors - Carlos Gorbea