NGFR/TNFRSF16 Polyklonaler Antikörper

NGFR/TNFRSF16 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(32)

Rezensionen (1)

Kat-Nr. : 55014-1-AP

Synonyme

NGFR, CD271, Gp80 LNGFR, Gp80-LNGFR, Low-affinity nerve growth factor receptor

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  WB analysis using 55014-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55014-1-AP (p75NTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55014-1-AP

  IP result of anti-p75NTR (IP:55014-1-AP, 3ug; Detection:55014-1-AP 1:300) with mouse brain tissue lysate 5000ug.

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  IHC staining of mouse brain using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55014-1-AP (p75NTR antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55014-1-AP (p75NTR antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 55014-1-AP (p75NTR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 55014-1-AP (p75NTR antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Prostatahyperplasie-Gewebe, humanes Milzgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 7 publications below
• CoIP: See 1 publications below

Produktinformation

55014-1-AP bindet in WB, IHC, IP, CoIP, ELISA NGFR/TNFRSF16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nerve growth factor receptor (TNFR superfamily, member 16)
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: NM_002507
• Gene symbol: p75NTR
• Gene ID (NCBI): 4804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.

Publikationen

Species	Application	Title
mouse		J Exp Med; CXCR4 regulates Plasmodium development in mouse and human hepatocytes.; Authors - Hironori Bando
mouse	WB	Int J Mol Sci; Direct Enhancement Effect of Hippocampal Cholinergic Neurostimulating Peptide on Cholinergic Activity in the Hippocampus; Authors - Yuta Madokoro
human	IHC	Int J Mol Sci; Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review; Authors - Hiroshi Toshida
mouse,human	WB,IHC	Front Oncol; LMO1 Plays an Oncogenic Role in Human Glioma Associated With NF-kB Pathway.; Authors - Lei Gao
human	WB	Stem Cell Res Ther; NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.; Authors - Ke Wu
human		J Mol Med (Berl); MESP2 variants contribute to conotruncal heart defects by inhibiting cardiac neural crest cell proliferation.; Authors - Erge Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Renato (Verified Customer) (02-27-2019)

Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue