NGFR/TNFRSF16 Rekombinanter Antikörper

NGFR/TNFRSF16 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242634F10

Kat-Nr. : 85622-2-RR

Synonyme

CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR

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  WB analysis using 85622-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85622-2-RR (Ngfr antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85622-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85622-2-RR (Ngfr antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using 85622-2-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using NGFR/TNFRSF16 antibody (85622-2-RR, Clone: 242634F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Maus-Uterusgewebe, Ratten-Uterusgewebe
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85622-2-RR bindet in WB, IF/ICC, ELISA NGFR/TNFRSF16 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NGFR/TNFRSF16 fusion protein Eg2651
• Vollständiger Name: nerve growth factor receptor (TNFR superfamily, member 16)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: NM_033217.3
• Gene symbol: Ngfr
• Gene ID (NCBI): 18053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

p75NTR, also named TNFRSF16, Gp80-LNGFR, p75 ICD, and CD271, is a low-affinity receptor that can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as the cell death of neural cells. The antibody is specific to NGFR.