NHE1 Polyklonaler Antikörper

NHE1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29761-1-PBS

Synonyme

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  WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29761-1-AP (same clone as 29761-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NHE1 antibody (29761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29761-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NHE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NHE1 fusion protein Ag31192
• Vollständiger Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_003047
• Gene symbol: NHE1
• Gene ID (NCBI): 6548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).