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NHE3 Polyklonaler Antikörper

NHE3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27190-1-PBS

Synonyme

SLC9A3, NHE-3

Filter:
  • Western Blot (WB) analysis of mouse small intestine tissue using NHE3 Polyclonal antibody (27190-1-AP)

    WB analysis of mouse small intestine using 27190-1-AP (same clone as 27190-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27190-1-AP (NHE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using NHE3 Polyclonal antibody (27190-1-AP)

    IP experiment of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    IP result of anti-NHE3 (IP:27190-1-AP, 4ug; Detection:27190-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NHE3 Polyclonal antibody (27190-1-AP)

    IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). Arrows show brush border. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NHE3 Polyclonal antibody (27190-1-AP)

    IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27190-1-PBS bindet in WB, IHC, IP, Indirect ELISA NHE3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NHE3 fusion protein Ag25637
Vollständiger Name solute carrier family 9 (sodium/hydrogen exchanger), member 3
Berechnetes Molekulargewicht 834 aa, 93 kDa
Beobachtetes Molekulargewicht 80-100 kDa
GenBank-ZugangsnummerBC101669
Gene symbol NHE3
Gene ID (NCBI) 6550
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC9A3, also known as NHE3, belongs to the sodium hydrogen exchange protein family (NHE) and is mainly responsible for regulating the concentration of sodium and hydrogen ions inside and outside the cell. SLC9A3 is a Major apical Na+/H+ exchanger in kidney and intestine, playing an important role in renal and intestine Na+ absorption and blood pressure regulation (PMID:24622516, 2635877). Mutations in SLC9A3 cause Congenital Sodium Diarrhea (PMID: 26358773, 31276831).