Entactin Polyklonaler Antikörper

Entactin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 13766-1-AP

Synonyme

NID1, NID, NID 1, NID-1, nidogen 1

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  WB analysis using 13766-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13766-1-AP (Entactin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13766-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13766-1-AP (Entactin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 13766-1-AP

  IP result of anti-Entactin (IP:13766-1-AP, 4ug; Detection:13766-1-AP 1:200) with human placenta tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 13766-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13766-1-AP (Entactin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13766-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Entactin antibody (13766-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Mausherzgewebe, Maus-Plazenta-Gewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13766-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Entactin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Entactin fusion protein Ag4749
• Vollständiger Name: nidogen 1
• Berechnetes Molekulargewicht: 1247 aa, 136 kDa
• Beobachtetes Molekulargewicht: 136-150 kDa
• GenBank-Zugangsnummer: BC045606
• Gene symbol: Entactin/NID
• Gene ID (NCBI): 4811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nidogen 1 (Entactin), first identified from a basement membrane-secreting cell line and the murine EHS tumor, is together with perlecan, laminin, and collagen IV, a ubiquitous component of basement membranes (PMID: 19530222). Nidogen-1 is made by mesenchymal cells and is incorporated into the epithelial BM. It is a sulfated glycoprotein of 150 kDa with three globular domains, G1, G2 and G3, and contains binding sites for other ECM molecules (PMID: 10958695, 10671374).

Publikationen

Species	Application	Title
rat	WB,CoIP	Bioact Mater; Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.; Authors - Pengzhen Cheng
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Biomaterials; Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs; Authors - Anna L Kersey
	WB	Signal Transduct Target Ther; Human adipose and umbilical cord mesenchymal stem cell-derived extracellular vesicles mitigate photoaging via TIMP1/Notch1; Authors - Huan Zhang