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NIPBL Polyklonaler Antikörper

NIPBL Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18792-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    WB analysis of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    WB analysis of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    IF Staining of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18792-1-AP (NIPBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

18792-1-PBS bindet in WB, IF/ICC, Indirect ELISA NIPBL und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NIPBL fusion protein Ag5663
Vollständiger Name Nipped-B homolog (Drosophila)
Berechnetes Molekulargewicht 2804 aa, 316 kDa
Beobachtetes Molekulargewicht 300 kDa
GenBank-ZugangsnummerBC033847
Gene symbol NIPBL
Gene ID (NCBI) 25836
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.