NIPSNAP1 Polyklonaler Antikörper

NIPSNAP1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33020-1-AP

Synonyme

Protein NipSnap homolog 1

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  WB analysis using 33020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33020-1-AP (NIPSNAP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, HepG2-Zellen, HuH-7-Zellen, Maushirngewebe, Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

33020-1-AP bindet in WB, ELISA NIPSNAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NIPSNAP1 fusion protein Ag36474
• Vollständiger Name: nipsnap homolog 1 (C. elegans)
• Berechnetes Molekulargewicht: 33kDa，284aa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_003634.3
• Gene symbol: NIPSNAP1
• Gene ID (NCBI): 8508
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NIPSNAP1 has been shown to play a role in regulating synaptic activity in learning and memory but nonetheless, along with the brain, NIPSNAP1 is highly abundant in other organs such as the liver, kidney, and adrenals but its expression is less evident in other tissues such as skeletal muscle. Functional investigations revealed the knockdown of NIPSNAP1 in cancer cells induced cell cycle arrest and inhibited proliferation with the underlying phenotype resulting from senescence. Further analyses revealed that NIPSNAP1 engages in two separate binding interactions that prevent cellular senescence. First, NIPSNAP1 engages with the E3 ubiquitin ligase FBXL14 which otherwise serves to target c-Myc for proteasomal degradation. And moreover, this was shown to be part of a regulatory feedback loop since NIPSNAP1 is subject to transcriptional repression by c-Myc. The second interaction involves NIPSNAP1 binding to superoxide dismutase 2 (SOD2) which enhances its association with SIRT3, thereby deacetylating SOD2 and increasing its activity, in turn, alleviating elevated ROS levels. Consequently, silencing NIPSNAP1 expression contributes to P27-mediated senescence via both decreasing the levels of c-Myc together with increasing ROS levels. Together these findings reveal an important role for NIPSNAP1 as a negative regulator of cancer cell senescence, which functions to sustain the viability of cancer cells under growth factor deprivation stress (PMID: 37340421).