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NIPSNAP1 Polyklonaler Antikörper

NIPSNAP1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33020-1-PBS

Synonyme

Protein NipSnap homolog 1

Filter:
  • Western Blot (WB) analysis of various lysates using NIPSNAP1 Polyclonal antibody (33020-1-AP)

    WB analysis using 33020-1-AP (same clone as 33020-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33020-1-AP (NIPSNAP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33020-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

33020-1-PBS bindet in WB, Indirect ELISA NIPSNAP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NIPSNAP1 fusion protein Ag36474
Vollständiger Name nipsnap homolog 1 (C. elegans)
Berechnetes Molekulargewicht33kDa，284aa
Beobachtetes Molekulargewicht30 kDa
GenBank-ZugangsnummerNM_003634.3
Gene symbol NIPSNAP1
Gene ID (NCBI) 8508
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NIPSNAP1 has been shown to play a role in regulating synaptic activity in learning and memory but nonetheless, along with the brain, NIPSNAP1 is highly abundant in other organs such as the liver, kidney, and adrenals but its expression is less evident in other tissues such as skeletal muscle. Functional investigations revealed the knockdown of NIPSNAP1 in cancer cells induced cell cycle arrest and inhibited proliferation with the underlying phenotype resulting from senescence. Further analyses revealed that NIPSNAP1 engages in two separate binding interactions that prevent cellular senescence. First, NIPSNAP1 engages with the E3 ubiquitin ligase FBXL14 which otherwise serves to target c-Myc for proteasomal degradation. And moreover, this was shown to be part of a regulatory feedback loop since NIPSNAP1 is subject to transcriptional repression by c-Myc. The second interaction involves NIPSNAP1 binding to superoxide dismutase 2 (SOD2) which enhances its association with SIRT3, thereby deacetylating SOD2 and increasing its activity, in turn, alleviating elevated ROS levels. Consequently, silencing NIPSNAP1 expression contributes to P27-mediated senescence via both decreasing the levels of c-Myc together with increasing ROS levels. Together these findings reveal an important role for NIPSNAP1 as a negative regulator of cancer cell senescence, which functions to sustain the viability of cancer cells under growth factor deprivation stress (PMID: 37340421).