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NIPSNAP3B Polyklonaler Antikörper

NIPSNAP3B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11789-1-AP

Synonyme

NIPSNAP3, NIPSNAP3B, Protein NipSnap homolog 3B, SNAP1

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using NIPSNAP3B Polyclonal antibody (11789-1-AP)

    WB analysis using 11789-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11789-1-AP (NIPSNAP3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NIPSNAP3B Polyclonal antibody (11789-1-AP)

    IHC staining of human lymphoma using 11789-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11789-1-AP (NIPSNAP3B antibody) at dilution of 1:50 (under 10x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslebergewebe, Rattenlebergewebe
Erfolgreiche Detektion in IHChumanes Lymphomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11789-1-AP bindet in WB, IHC, ELISA NIPSNAP3B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NIPSNAP3B fusion protein Ag2377
Vollständiger Name nipsnap homolog 3B (C. elegans)
Berechnetes Molekulargewicht 247 aa, 28 kDa
Beobachtetes Molekulargewicht 28-30 kDa
GenBank-ZugangsnummerBC017914
Gene symbol NIPSNAP3B
Gene ID (NCBI) 55335
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NIPSNAP3B is a member of the NIPSNAP family with putative roles in vesicular trafficking (PMID: 15177564). The gene of NIPSNAP3B maps to chromosome 9q31.3, and encodes a 247-amino-acid protein with a calculated molecular mass of 28 kDa. NIPSNAP3B gene has been reported to be highly associated with low bone mineral density (BMD) variation in postmenopausal Caucasian women (PMID: 19922823).

Protokolle

Produktspezifische Protokolle
WB protocol for NIPSNAP3B antibody 11789-1-APProtokoll herunterladen
IHC protocol for NIPSNAP3B antibody 11789-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kristina (Verified Customer) (01-21-2022)

Beside NIPSNAP3A, I also tested an antibody for NIPSNAP3B as I study these proteins and their relevance in pathophysiology of Parkinson's disease for my PhD thesis. Also the NIPSNAP3B antibody showed a very precise result with one clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: LUHMES