NIT1 Polyklonaler Antikörper

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NIT1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14380-1-PBS

Synonyme

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  WB analysis using 14380-1-AP (same clone as 14380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293T using 14380-1-AP (same clone as 14380-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14380-1-AP (same clone as 14380-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14380-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 14380-1-AP (same clone as 14380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14380-1-AP (same clone as 14380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14380-1-AP (same clone as 14380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14380-1-AP (same clone as 14380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14380-1-AP (same clone as 14380-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14380-1-AP (NIT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14380-1-PBS bindet in WB, IHC, IF/ICC, ELISA NIT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NIT1 fusion protein Ag5764
• Vollständiger Name: nitrilase 1
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 32-36 kDa
• GenBank-Zugangsnummer: BC046149
• Gene symbol: NIT1
• Gene ID (NCBI): 4817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.