NIT2 Monoklonaler Antikörper

NIT2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E5B1

Kat-Nr. : 68169-1-PBS

Synonyme

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

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  WB analysis of HeLa using 68169-1-Ig (same clone as 68169-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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  WB analysis using 68169-1-Ig (same clone as 68169-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 68169-1-Ig (same clone as 68169-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 68169-1-Ig (same clone as 68169-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 68169-1-Ig (same clone as 68169-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68169-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NIT2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NIT2 fusion protein Ag9889
• Vollständiger Name: nitrilase family, member 2
• Berechnetes Molekulargewicht: 276 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC020620
• Gene symbol: NIT2
• Gene ID (NCBI): 56954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.