NK1.1 (CD161) Monoklonaler Antikörper

NK1.1 (CD161) Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen(2)

CloneNo.

PK136

Kat-Nr. : PE-65138

Synonyme

Klrb1a, CD161a, PK136, Nkrp1a, NK1.1

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  FC experiment of mouse splenocytes using PE-65138

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse NK1.1 (CD161) (PK136) (PE-65138, Clone:PK136) or 0.2 ug PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

PE-65138 bindet in FC NK1.1 (CD161) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NK-1+-Zellen aus Mausmilz und -knochenmark
• Vollständiger Name: killer cell lectin-like receptor subfamily B member 1A
• GenBank-Zugangsnummer: BC120707
• Gene symbol: NK1.1
• Gene ID (NCBI): 17057
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

NK1.1 (CD161), also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

Publikationen

Species	Application	Title
mouse	FC	Nat Nanotechnol; A bispecific nanosystem activates endogenous natural killer cells in the bone marrow for haematologic malignancies therapy; Authors - Yanqin Zhang
mouse	FC	Adv Sci (Weinh); EGR3 Inhibits Tumor Progression by Inducing Schwann Cell-Like Differentiation; Authors - Cai-Hong Chen