NKCC1/SLC12A2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NKCC1/SLC12A2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13884-1-PBS

Synonyme

NKCC1,SLC12A2, NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter

---

• 

  WB analysis using 13884-1-AP (same clone as 13884-1-PBS)

  Mouse brain total extract lysate and 37°C incubated mouse brain membrane extract lysate were subjected to SDS PAGE followed by western blot with 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 13884-1-AP (same clone as 13884-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NKCC1/SLC12A2 antibody (13884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

13884-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NKCC1/SLC12A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NKCC1/SLC12A2 fusion protein Ag4991
• Vollständiger Name: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC033003
• Gene symbol: NKCC1/SLC12A2
• Gene ID (NCBI): 6558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).