NKCC1/SLC12A2 Rekombinanter Antikörper

NKCC1/SLC12A2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250829D8

Kat-Nr. : 86201-1-RR

Synonyme

NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter, BSC

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  WB analysis using 86201-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 86201-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 86201-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 86201-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 86201-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86201-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86201-1-RR against Human NKCC1/SLC12A2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bei 37°C inkubiertes Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86201-1-RR bindet in WB, IHC, ELISA NKCC1/SLC12A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NKCC1/SLC12A2 fusion protein Ag4991
• Vollständiger Name: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC033003
• Gene symbol: NKCC1/SLC12A2
• Gene ID (NCBI): 6558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).