NKD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NKD2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16699-1-AP

Synonyme

Naked-2, Naked2, Naked 2, hNkd2

---

• 

  WB analysis of mouse kidney using 16699-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16699-1-AP

  WB result of NKD2 antibody (16699-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKD2 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 16699-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 16699-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, Maus-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16699-1-AP bindet in WB, IP, CoIP, ELISA NKD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NKD2 fusion protein Ag10094
• Vollständiger Name: naked cuticle homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC012176
• Gene symbol: NKD2
• Gene ID (NCBI): 85409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).

Publikationen

Species	Application	Title
human	WB	Oncogene; NKD2, a negative regulator of Wnt signaling, suppresses tumor growth and metastasis in osteosarcoma.; Authors - S Zhao ; KD Validated
human	WB	Biosci Biotechnol Biochem; Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells.; Authors - Weiran Xu
human	WB	Kaohsiung J Med Sci; Curcumin suppresses the Wnt/β-catenin signaling pathway by inhibiting NKD2 methylation to ameliorate intestinal ischemia/reperfusion injury; Authors - Jia-Xi Zhu
human	WB,IP,CoIP	Rejuvenation Res; Tripartite Motif-Containing Protein 65 Promotes Proliferation and Inhibits Ferroptosis in Prostate Cancer via Enhancing NKD Inhibitor of WNT Signaling Pathway 2 Ubiquitination; Authors - Chengcai Wang