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NKD2 Polyklonaler Antikörper

NKD2 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16699-1-PBS

Synonyme

Naked-2, Naked2, Naked 2, hNkd2

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using NKD2 Polyclonal antibody (16699-1-AP)

    WB analysis of mouse kidney using 16699-1-AP (same clone as 16699-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NKD2 Polyclonal antibody (16699-1-AP)

    WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

    WB result of NKD2 antibody (16699-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NKD2 Polyclonal antibody (16699-1-AP)

    WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using NKD2 Polyclonal antibody (16699-1-AP)

    WB analysis of mouse colon using 16699-1-AP (same clone as 16699-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

16699-1-PBS bindet in WB, Indirect ELISA NKD2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NKD2 fusion protein Ag10094
Vollständiger Name naked cuticle homolog 2 (Drosophila)
Berechnetes Molekulargewicht 50 kDa
Beobachtetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerBC012176
Gene symbol NKD2
Gene ID (NCBI) 85409
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).