NKX2-5 Polyklonaler Antikörper

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• KD/KO Validated

NKX2-5 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13921-1-PBS

Synonyme

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  WB analysis of human heart using 13921-1-AP (same clone as 13921-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 13921-1-AP (same clone as 13921-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 13921-1-AP (same clone as 13921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 13921-1-AP (same clone as 13921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse embryo using 13921-1-AP (same clone as 13921-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using NKX2-5 antibody (13921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13921-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA NKX2-5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NKX2-5 fusion protein Ag4930
• Vollständiger Name: NK2 transcription factor related, locus 5 (Drosophila)
• Berechnetes Molekulargewicht: 324 aa, 35 kDa
• Beobachtetes Molekulargewicht: 30-42 kda
• GenBank-Zugangsnummer: BC025711
• Gene symbol: NKX2-5
• Gene ID (NCBI): 1482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].