NKX3-1 Polyklonaler Antikörper

NKX3-1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 13069-1-AP

Synonyme

BAPX2, Homeobox protein Nkx 3.1, NK3 homeobox 1, NKX3, NKX3 1, NKX3.1, NKX3-1, NKX3A

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  WB analysis of LNCaP using 13069-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13069-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

13069-1-AP bindet in WB, IHC, ELISA NKX3-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NKX3-1 fusion protein Ag4070
• Vollständiger Name: NK3 homeobox 1
• Berechnetes Molekulargewicht: 234 aa, 26 kDa
• Beobachtetes Molekulargewicht: 34-38 kDa
• GenBank-Zugangsnummer: BC074864
• Gene symbol: NKX3-1
• Gene ID (NCBI): 4824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)

Publikationen

Species	Application	Title
human	IHC	bioRxiv; ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity; Authors - Weiran Feng
mouse	IHC	Sci Rep; Prostatic androgen receptor signaling shows an age-related and lobe-specific alteration in mice; Authors - Han Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parmveer (Verified Customer) (08-28-2019)

Worked well for both immunohistochemistry and immunofluorescence. FFPE prostate tissue used, primary applied for 1 hour at room temperature. There is a bit of background that is hard to avoid, but luminal prostate nuclei are clearly marked.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Prostate

NKX3-1 Antibody Immunohistochemistry,Immunofluorescence, validation (1:500 dilution) in Prostate (Cat no:13069-1-AP)