NKX3-1 Polyklonaler Antikörper

NKX3-1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13069-1-PBS

Synonyme

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  WB analysis of human testis using 13069-1-AP (same clone as 13069-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13069-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 13069-1-AP (same clone as 13069-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13069-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13069-1-AP (same clone as 13069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13069-1-AP (same clone as 13069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13069-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13069-1-PBS bindet in WB, IHC, Indirect ELISA NKX3-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NKX3-1 fusion protein Ag4070
• Vollständiger Name: NK3 homeobox 1
• Berechnetes Molekulargewicht: 234 aa, 26 kDa
• Beobachtetes Molekulargewicht: 34-38 kDa
• GenBank-Zugangsnummer: BC074864
• Gene symbol: NKX3-1
• Gene ID (NCBI): 4824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)