NKX3-1 Rekombinanter Antikörper

NKX3-1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250402B8

Kat-Nr. : 86085-1-RR

Synonyme

BAPX2, Homeobox protein NK-3 homolog A, Homeobox protein Nkx 3.1, Homeobox protein Nkx-3.1, NK3 homeobox 1

---

• 

  WB analysis of LNCaP using 86085-1-RR

  LNCaP cells were subjected to SDS PAGE followed by western blot with 86085-1-RR (NKX3-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86085-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86085-1-RR against Human NKX3-1 were performed. The affinity constant is 0.411 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86085-1-RR bindet in WB, ELISA NKX3-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NKX3-1 fusion protein Ag4070
• Vollständiger Name: NK3 homeobox 1
• Berechnetes Molekulargewicht: 234 aa, 26 kDa
• Beobachtetes Molekulargewicht: 34-38 kDa
• GenBank-Zugangsnummer: BC074864
• Gene symbol: NKX3-1
• Gene ID (NCBI): 4824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)