NKp46/NCR1 Rekombinanter Antikörper

NKp46/NCR1 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241811H7

Kat-Nr. : 84416-5-PBS

Synonyme

241811H7, Activating receptor 1, Ar1, CD335, Kilr1

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  WB analysis of mouse splenocytes using 84416-5-RR (same clone as 84416-5-PBS)

  mouse splenocytes were subjected to SDS PAGE followed by western blot with 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 84416-5-RR (same clone as 84416-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 84416-5-RR (same clone as 84416-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  IHC staining of rat thymus using 84416-5-RR (same clone as 84416-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  IF Staining of rat spleen using 84416-5-RR (same clone as 84416-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84416-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84416-5-RR against Rat NKp46/NCR1 were performed. The affinity constant is 0.593 nM.

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Geprüfte Anwendungen

Produktinformation

84416-5-PBS bindet in WB, IHC, IF-P, Indirect ELISA NKp46/NCR1 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NKp46/NCR1 fusion protein Eg1469
• Vollständiger Name: natural cytotoxicity triggering receptor 1
• Berechnetes Molekulargewicht: 37kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: NM_057199.1
• Gene symbol: NKp46/NCR1
• Gene ID (NCBI): 117547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).