NLGN1 Monoklonaler Antikörper

NLGN1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D1A12

Kat-Nr. : 66964-1-PBS

Synonyme

NLG1, KIAA1070, neuroligin 1, Neuroligin-1

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  WB analysis of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66964-1-Ig (same clone as 66964-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 66964-1-Ig (same clone as 66964-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66964-1-Ig (same clone as 66964-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66964-1-Ig (same clone as 66964-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66964-1-PBS bindet in WB, IHC, Indirect ELISA NLGN1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NLGN1 fusion protein Ag6356
• Vollständiger Name: neuroligin 1
• Berechnetes Molekulargewicht: 840 aa, 94 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC032555
• Gene symbol: NLGN1
• Gene ID (NCBI): 22871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NLGN1, also named KIAA1070, belongs to the type-B carboxylesterase/lipase family. Neuroligin1 (NLGN1) is a main component of the excitatory glutamatergic synapses complex and plays a role in synapse assembly and function (PMID: 34340665). NLGN1 has 2 isoforms with the MV of 92 and 96 kDa. Sometimes higher molecular weight of around 116 kDa can also be observed, which is a glycosylated mature form of NLGN1 (PMID: 28841651).