NLGN3 Polyklonaler Antikörper

NLGN3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (2)

Kat-Nr. : 29319-1-AP

Synonyme

NL3, Neuroligin-3, neuroligin 3, HNL3, AUTSX1

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  WB analysis using 29319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29319-1-AP (NLGN3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29319-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29319-1-AP (NLGN3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29319-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29319-1-AP (NLGN3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29319-1-AP bindet in WB, IHC, ELISA NLGN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NLGN3 fusion protein Ag30536
• Vollständiger Name: neuroligin 3
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC051715
• Gene symbol: NLGN3
• Gene ID (NCBI): 54413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuroligin3 (NLGN3) belongs to the neuroligin family, a class of postsynaptic cell adhesion molecules that regulate synapse organization and dendritic outgrowth, and NLGN3 missense variants have previously been associated with autistic spectrum disorder (ASD).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (07-23-2025)

Used for WB and 1:1000 dilution. Detected a clear band at around 75 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells

FH

Wojciech (Verified Customer) (07-10-2025)

The antibody was used for Western blotting in cell lysates. A prominent band was observed at the ~75 kDa. While some non-specific bands were present ~60 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: T cells