NLRC5 Rekombinanter Antikörper

NLRC5 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240338D8

Kat-Nr. : 83384-2-PBS

Synonyme

240338D8, Caterpiller protein 16.1, CLR16.1, NOD27, NOD4

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  IF Staining of THP-1 using 83384-2-RR (same clone as 83384-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NLRC5 antibody (83384-2-RR, Clone: 240338D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83384-2-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 83384-2-RR (same clone as 83384-2-PBS)

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug NLRC5 Recombinant antibody (83384-2-RR, Clone:240338D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83384-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00402-3

  Cytometric bead array standard curve of MP00402-3, NLRC5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83384-2-PBS. Detection antibody: 83384-3-PBS. Standard: Ag34508. Range: 1.56-100 ng/mL.

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  Affinity and Kinetic Characterization of 83384-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83384-2-RR against Human NLRC5 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83384-2-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA NLRC5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NLRC5 fusion protein Ag34508
• Vollständiger Name: NLR family, CARD domain containing 5
• Berechnetes Molekulargewicht: 204 kDa
• GenBank-Zugangsnummer: BC063566
• Gene symbol: NLRC5
• Gene ID (NCBI): 84166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The nucleotide-binding domain leucine-rich repeat containing (NLR) family of proteins is mainly involved in microbial pathogen recognition, inflammatory responses, and cell death. NLRC5, the largest member of the NLR family, is currently receiving an increasing level of attention. NLRC5 plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways.