NLRP12 Rekombinanter Antikörper

NLRP12 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241838E11

Kat-Nr. : 83123-6-RR

Synonyme

PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1, Monarch 1

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  WB analysis using 83123-6-RR

  Mouse liver tissue was subjected to SDS PAGE followed by western blot with 83123-6-RR (NLRP12 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 83123-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83123-6-RR (NLRP12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 83123-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83123-6-RR (NLRP12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83123-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83123-6-RR against Human NLRP12 were performed. The affinity constant is 0.236 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83123-6-RR bindet in WB, ELISA NLRP12 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NLRP12 fusion protein Ag34281
• Vollständiger Name: NLR family, pyrin domain containing 12
• Berechnetes Molekulargewicht: 1061 aa, 120 kDa
• Beobachtetes Molekulargewicht: 95-110 kDa
• GenBank-Zugangsnummer: BC028069
• Gene symbol: NLRP12
• Gene ID (NCBI): 91662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).