NLRP2 Polyklonaler Antikörper

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• KD/KO Validated

NLRP2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 15182-1-AP

Synonyme

CLR19.9, NACHT, LRR and PYD domains-containing protein 2, NALP2, NBS1, Nucleotide-binding site protein 1

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  WB analysis of A549 using 15182-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15182-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 15182-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 15182-1-AP

  IP result of anti-NLRP2 (IP:15182-1-AP, 4ug; Detection:15182-1-AP 1:3000) with A549 cells lysate 920 ug.

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  IHC staining of human placenta using 15182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15182-1-AP (NLRP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15182-1-AP (NLRP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Lungengewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

15182-1-AP bindet in WB, IHC, IF, IP, ELISA NLRP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NLRP2 fusion protein Ag7167
• Vollständiger Name: NLR family, pyrin domain containing 2
• Berechnetes Molekulargewicht: 121 kDa
• Beobachtetes Molekulargewicht: 105-110 kDa
• GenBank-Zugangsnummer: BC001039
• Gene symbol: NLRP2
• Gene ID (NCBI): 55655
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Front Immunol; Macrophage IL-1β contributes to tumorigenesis through paracrine AIM2 inflammasome activation in the tumor microenvironment; Authors - Zhi Huan Chew
mouse	WB,IHC	Brain Behav Immun; Kynurenine regulates NLRP2 inflammasome in astrocytes and its implications in depression.; Authors - Qingyu Zhang ; KD Validated
human	WB	Eur J Immunol; NLRP2 negatively regulates antiviral immunity by interacting with TBK1.; Authors - Yanqing Yang ; KD Validated
human	IHC	Front Microbiol; Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.; Authors - Pei-Hui Ding
mouse	IHC	Mol Cell Endocrinol; Glucagon-like peptide-1 analogs mitigate neuroinflammation in Alzheimer's disease by suppressing NLRP2 activation in astrocytes; Authors - Mengjun Zhang
mouse	WB	J Neuroimmunol; A selective NLRP3 inflammasome inhibitor attenuates behavioral deficits and neuroinflammation in a mouse model of Parkinson's disease.; Authors - Shuxuan Huang