NLRP3 Polyklonaler Antikörper

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• KD/KO Validated

NLRP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27458-1-PBS

Synonyme

Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, CIAS1, Cold-induced autoinflammatory syndrome 1 protein, EC:3.6.4.-

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  WB analysis using 27458-1-AP (same clone as 27458-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27458-1-AP (NLRP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 27458-1-AP (same clone as 27458-1-PBS)

  WB result of NLRP3 antibody (27458-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NLRP3 transfected THP-1 cells. This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

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  IP experiment of THP-1 using 27458-1-AP (same clone as 27458-1-PBS)

  IP result of anti-NLRP3 (IP:27458-1-AP, 4ug; Detection:27458-1-AP 1:800) with THP-1 cells lysate 1520 ug. This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27458-1-AP (same clone as 27458-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27458-1-AP (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27458-1-AP (same clone as 27458-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27458-1-AP (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 27458-1-AP (same clone as 27458-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody (27458-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27458-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NLRP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NLRP3 fusion protein Ag26272
• Vollständiger Name: NLR family, pyrin domain containing 3
• Berechnetes Molekulargewicht: 118 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_001079821
• Gene symbol: NLRP3
• Gene ID (NCBI): 114548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death(PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa.