NLRP3 Monoklonaler Antikörper

NLRP3 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3H1A7

Kat-Nr. : 68102-1-Ig

Synonyme

AGTAVPRL, AII, AII/AVP, AVP, C1orf7, Caterpiller protein 1.1, CIAS1, CLR1.1, Cryopyrin, FCAS, FCU, FLJ95925, MWS, NALP3, NLRP3, PYPAF1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBTHP-1-Zellen, mit LPS behandelte THP-1-Zellen, mit LPS behandelte RAW 264.7-Zellen, RAW 264.7-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

68102-1-Ig bindet in WB, IHC, IF, ELISA NLRP3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen NLRP3 fusion protein Ag26289
Vollständiger Name NLR family, pyrin domain containing 3
Berechnetes Molekulargewicht 118 kDa
Beobachtetes Molekulargewicht110 kDa
GenBank-ZugangsnummerNM_001079821
Gene symbol NLRP3
Gene ID (NCBI) 114548
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID).

Protokolle

Produktspezifische Protokolle
WB protocol for NLRP3 antibody 68102-1-IgProtokoll herunterladen
IHC protocol for NLRP3 antibody 68102-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

Aristolochic acid I exposure triggers ovarian dysfunction by activating NLRP3 inflammasome and affecting mitochondrial homeostasis

Authors - Ming-Xin Sun
mouseWB

Oxid Med Cell Longev

Hydrogen Sulfide Ameliorated High Choline-Induced Cardiac Dysfunction by Inhibiting cGAS-STING-NLRP3 Inflammasome Pathway

Authors - Lu Bai
humanWB

Front Pharmacol

Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway

Authors - Xiaowei Sun
mouse,ratWB

Int J Mol Sci

Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway

Authors - Pan Lei
mouseWB

Bioorg Chem

Fusion of Michael-acceptors enhances the anti-inflammatory activity of ginsenosides as potential modulators of the NLRP3 signaling pathway

Authors - Gangqiang Yang
mouseWB,IF

Arterioscler Thromb Vasc Biol

Sleep Deprivation Promotes Endothelial Inflammation and Atherogenesis by Reducing Exosomal miR-182-5p

Authors - Xiao Li