NLRP3 Monoklonaler Antikörper
NLRP3 Monoklonal Antikörper für IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
3H1A7
Kat-Nr. : 68102-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | THP-1-Zellen, mit LPS behandelte THP-1-Zellen, mit LPS behandelte RAW 264.7-Zellen, RAW 264.7-Zellen |
Erfolgreiche Detektion in IHC | humanes Nierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 18 publications below |
IHC | See 5 publications below |
IF | See 4 publications below |
Produktinformation
68102-1-Ig bindet in WB, IHC, IF, ELISA NLRP3 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | NLRP3 fusion protein Ag26289 |
Vollständiger Name | NLR family, pyrin domain containing 3 |
Berechnetes Molekulargewicht | 118 kDa |
Beobachtetes Molekulargewicht | 110 kDa |
GenBank-Zugangsnummer | NM_001079821 |
Gene symbol | NLRP3 |
Gene ID (NCBI) | 114548 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID).
Protokolle
Produktspezifische Protokolle | |
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WB protocol for NLRP3 antibody 68102-1-Ig | Protokoll herunterladen |
IHC protocol for NLRP3 antibody 68102-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Free Radic Biol Med Aristolochic acid I exposure triggers ovarian dysfunction by activating NLRP3 inflammasome and affecting mitochondrial homeostasis | ||
Oxid Med Cell Longev Hydrogen Sulfide Ameliorated High Choline-Induced Cardiac Dysfunction by Inhibiting cGAS-STING-NLRP3 Inflammasome Pathway | ||
Front Pharmacol Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway | ||
Int J Mol Sci Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway | ||
Bioorg Chem Fusion of Michael-acceptors enhances the anti-inflammatory activity of ginsenosides as potential modulators of the NLRP3 signaling pathway | ||
Arterioscler Thromb Vasc Biol Sleep Deprivation Promotes Endothelial Inflammation and Atherogenesis by Reducing Exosomal miR-182-5p |