NLRP3 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NLRP3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(163)

CloneNo.

3H1A7

Rezensionen (1)

Kat-Nr. : 68102-1-Ig

Synonyme

3H1A7, Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, CIAS1, Cold-induced autoinflammatory syndrome 1 protein

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• 

  WB analysis using 68102-1-Ig

  Non-treated THP-1 cells and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

• 

  IHC staining of human kidney using 68102-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 68102-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, mit LPS behandelte THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 130 publications below
• IHC: See 30 publications below
• IF: See 47 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

68102-1-Ig bindet in WB, IHC, IF, IP, CoIP, ELISA, Cell treatment NLRP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Rind, Zebrafisch
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NLRP3 fusion protein Ag26289
• Vollständiger Name: NLR family, pyrin domain containing 3
• Berechnetes Molekulargewicht: 118 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_001079821
• Gene symbol: NLRP3
• Gene ID (NCBI): 114548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa(PMID: 17164409, 34680443).

Publikationen

Species	Application	Title
	WB	Phytomedicine; Artesunate ameliorates diabetic xerostomia in rats through regulating oral microbiota and metabolic profile in salivary gland based on NF-κB/NLRP3 signaling pathway; Authors - Siqin Zhang
	WB	Phytomedicine; Geniposide via enema alleviates colitis by modulating intestinal flora and bile acid metabolites, inhibiting S100A8/S100A9/NF-κB, and promoting TGR5 inhibition of NLRP3 inflammasome; Authors - Bao-Xin Zheng
	WB	Free Radic Biol Med; Aristolochic acid I exposure triggers ovarian dysfunction by activating NLRP3 inflammasome and affecting mitochondrial homeostasis; Authors - Ming-Xin Sun
	WB	Oxid Med Cell Longev; Hydrogen Sulfide Ameliorated High Choline-Induced Cardiac Dysfunction by Inhibiting cGAS-STING-NLRP3 Inflammasome Pathway; Authors - Lu Bai
human	IF	Front Immunol; USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis; Authors - Chenye Zhao
human	WB,IP	Biochim Biophys Acta Mol Basis Dis; Ischemia-reperfusion injury induces ZBP1-dependent PANoptosis in endothelial cells; Authors - Yue Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-19-2024)

Worked fine.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCM