NLRP3 Monoklonaler Antikörper

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• KD/KO Validated

NLRP3 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

3H1A7

Kat-Nr. : 68102-1-PBS

Synonyme

3H1A7, Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, CIAS1, Cold-induced autoinflammatory syndrome 1 protein

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  WB analysis using 68102-1-Ig (same clone as 68102-1-PBS)

  Non-treated THP-1 cells and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 68102-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50203-1

  Sandwich ELISA standard curve of MP50203-1, Human NLRP3 Monoclonal Matched Antibody Pair - PBS only. 68102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26289. 68102-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP50203-1

  The mean NLRP3 concentration was determined to be 27.37 ng/mL in THP-1 cell extract based on a 1.50 mg/mL extract load.

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  IHC staining of human kidney using 68102-1-Ig (same clone as 68102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68102-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68102-1-Ig (same clone as 68102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68102-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50203-1

  Cytometric bead array standard curve of MP50203-1, NLRP3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68102-1-PBS. Detection antibody: 68102-2-PBS. Standard:Ag26289. Range: 1.563-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68102-1-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test NLRP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NLRP3 fusion protein Ag26289
• Vollständiger Name: NLR family, pyrin domain containing 3
• Berechnetes Molekulargewicht: 118 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_001079821
• Gene symbol: NLRP3
• Gene ID (NCBI): 114548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa(PMID: 17164409, 34680443).