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NLRP3 Rekombinanter Antikörper

NLRP3 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250083C8

Kat-Nr. : 85967-1-PBS

Synonyme

AGTAVPRL, AII/AVP, Mmig1, MWS, NALP3

Filter:
  • Western Blot (WB) analysis of various lysates using NLRP3 Recombinant monoclonal antibody (85967-1-RR)

    WB analysis using 85967-1-RR (same clone as 85967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85967-1-RR (NLRP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85967-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NLRP3 Recombinant monoclonal antibody (85967-1-RR)

    WB analysis using 85967-1-RR (same clone as 85967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85967-1-RR (NLRP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85967-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85967-1-PBS bindet in WB, Indirect ELISA NLRP3 und zeigt Reaktivität mit Maus, Ratten

Getestete Reaktivität Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NLRP3 fusion protein Ag32515
Vollständiger Name NLR family, pyrin domain containing 3
Berechnetes Molekulargewicht118kd
Beobachtetes Molekulargewicht108-120 kDa
GenBank-ZugangsnummerNM_145827
Gene symbol Nlrp3
Gene ID (NCBI) 216799
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse NLRP3.