NLRP6 Polyklonaler Antikörper

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• KD/KO Validated

NLRP6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30973-1-PBS

Synonyme

NALP6, PYPAF5

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  WB analysis using 30973-1-AP (same clone as 30973-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30973-1-AP (NLRP6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse colon using 30973-1-AP (same clone as 30973-1-PBS)

  IP result of anti-NLRP6 (IP:30973-1-AP, 4ug; Detection:30973-1-AP 1:2000) with mouse colon tissue lysate 760 ug. This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 30973-1-AP (same clone as 30973-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 30973-1-AP (NLRP6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 30973-1-AP (same clone as 30973-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NLRP6 antibody (30973-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30973-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NLRP6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NLRP6 fusion protein Ag34516
• Vollständiger Name: NLR family, pyrin domain containing 6
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 98-105 kDa
• GenBank-Zugangsnummer: NM_138329.2
• Gene symbol: NLRP6
• Gene ID (NCBI): 171389
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOD-like receptor family pyrin domain containing 6 (NLRP6) is also named as NACHT, LRR and PYD domains-containing protein 6 and ZFML, Angiotensin II/vasopressin receptor, NALP6 and PYPAF5. NLRP6 forms a complex with ASC and caspase-1 or caspase-11 to form an inflammasome complex cleaving pro-interleukin-1β (IL-1β) and IL-18 into their biologically active forms. The function of NLRP6 has been attributed to the maintenance of epithelial integrity and host defense against microbial infections (PMID: 33910012). NLRP6 is reported to involved in inflammasome activation, regulation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, antiviral interferon (IFN) signaling, mucus secretion, and antimicrobial peptide (AMP) production. NLRP6 is a novel NLR family member, that shows high expression in the intestine and liver (in contrast to NLRP3 in myeloid cells), to regulate inflammation and host defense against microbes (PMID: 32386845).