NMDAR1/GRIN1 Polyklonaler Antikörper

NMDAR1/GRIN1 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27232-1-PBS

Synonyme

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  WB analysis using 27232-1-AP (same clone as 27232-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 27232-1-AP (same clone as 27232-1-PBS)

  IP result of anti-NMDAR1/GRIN1 (IP:27232-1-AP, 4ug; Detection:27232-1-AP 1:400) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 27232-1-AP (same clone as 27232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 27232-1-AP (same clone as 27232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 27232-1-AP (same clone as 27232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 27232-1-AP (same clone as 27232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1/GRIN1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 27232-1-AP (same clone as 27232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1/GRIN1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27232-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA NMDAR1/GRIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMDAR1/GRIN1 fusion protein Ag26093
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 116-120 kDa
• GenBank-Zugangsnummer: NM_000832
• Gene symbol: NMDAR1
• Gene ID (NCBI): 2902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).