NMDAR1/GRIN1 Rekombinanter Antikörper

NMDAR1/GRIN1 Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

250535B1

Kat-Nr. : 85973-3-RR

Synonyme

GRIN1, NMDAR1, GluN1, Glutamate receptor ionotropic, NMDA 1, NMDAR 1

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  WB analysis using 85973-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85973-3-RR (NMDAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 85973-3-RR

  IP result of anti-NMDAR1/GRIN1 (IP:85973-3-RR, 4ug; Detection:85973-3-RR 1:1000) with mouse brain tissue lysate 1720 ug.

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  Affinity and Kinetic Characterization of 85973-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85973-3-RR against Human NMDAR1/GRIN1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85973-3-RR bindet in WB, IP, ELISA NMDAR1/GRIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NMDAR1/GRIN1 fusion protein Ag26093
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 116-120 kDa
• GenBank-Zugangsnummer: NM_000832
• Gene symbol: NMDAR1
• Gene ID (NCBI): 2902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).