NMDAR2A/GRIN2A Polyklonaler Antikörper

NMDAR2A/GRIN2A Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 28571-1-AP

Synonyme

GRIN2A, NMDAR2A, glun2a, Glutamate [NMDA] receptor subunit epsilon-1, Glutamate receptor ionotropic, NMDA 2A

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  WB analysis using 28571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

28571-1-AP bindet in WB, IHC, IF-P, IF-Fro, ELISA NMDAR2A/GRIN2A und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMDAR2A/GRIN2A fusion protein Ag29423
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Berechnetes Molekulargewicht: 165 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: NM_000833
• Gene symbol: GRIN2A
• Gene ID (NCBI): 2903
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Behav Brain Res; Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice; Authors - Shukun Jiang
rat	WB	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang
mouse	WB	Neurochem Res; Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression; Authors - Yunyi Huang
mouse	WB	J Ethnopharmacol; Anticonvulsant effect of Stachydrine on pentylenetetrazole-induced kindling seizure mouse model via Notch and NMDAR signaling pathways; Authors - Qifa Li
mouse	IF	Neuron; The paraventricular thalamus mediates visceral pain and anxiety-like behaviors via two distinct pathways; Authors - Di Li