NME2 Polyklonaler Antikörper

NME2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20493-1-PBS

Synonyme

C-myc purine-binding transcription factor PUF, EC:2.7.13.3, EC:2.7.4.6, Histidine protein kinase NDKB, NDKB

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  WB analysis of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 20493-1-AP (same clone as 20493-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 20493-1-AP (same clone as 20493-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20493-1-AP (NME2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 20493-1-AP (same clone as 20493-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20493-1-AP (NME2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20493-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NME2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NME2 fusion protein Ag13913
• Vollständiger Name: non-metastatic cells 2, protein (NM23B) expressed in
• Berechnetes Molekulargewicht: 152 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC002476
• Gene symbol: NME2
• Gene ID (NCBI): 4831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B) and NM23B, and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates, supplying nucleotide pools for DNA/RNA synthesis, and interacts with membrane receptors and constitutes a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.