NME5 Polyklonaler Antikörper

NME5 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12923-1-AP

Synonyme

IPIA beta, NDK H 5, NDP kinase homolog 5, NME5, Testis specific nm23 homolog

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12923-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12923-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12923-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12923-1-AP

  IP result of anti-NME5 (IP:12923-1-AP, 4ug; Detection:12923-1-AP 1:500) with mouse testis tissue lysate 3920 ug.

• 

  IHC staining of mouse testis using 12923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12923-1-AP (NME5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12923-1-AP (NME5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Nierengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

12923-1-AP bindet in WB, IP, IHC, IF, ELISA NME5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NME5 fusion protein Ag3610
• Vollständiger Name: non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase)
• Berechnetes Molekulargewicht: 212 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC026182
• Gene symbol: NME5
• Gene ID (NCBI): 8382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NME5(Nucleoside diphosphate kinase homolog 5) belongs to the NDK family. Its expression plays a critical role in spermiogenesis by increasing the cellular levels of GPX5 to eliminate reactive oxygen species. NME5 may have specific functions in the phosphotransfer network involved in spermiogenesis and flagellar movement.

Publikationen

Species	Application	Title
mouse	WB,IF	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB	J Hazard Mater; TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.; Authors - Changying Li
human	IHC	Int J Cancer; Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma.; Authors - Parris Toshima Z TZ
mouse	WB,IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma