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NNMT Polyklonaler Antikörper
NNMT Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15123-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | L02-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe | 
| Erfolgreiche IP | HepG2-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Magenkrebsgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:400-1:1600 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:20-1:200 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 7 publications below | 
| WB | See 22 publications below | 
| IHC | See 8 publications below | 
| IF | See 2 publications below | 
| IP | See 1 publications below | 
Produktinformation
15123-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA NNMT und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | NNMT fusion protein Ag7197 | 
| Vollständiger Name | nicotinamide N-methyltransferase | 
| Berechnetes Molekulargewicht | 30 kDa | 
| Beobachtetes Molekulargewicht | 30 kDa | 
| GenBank-Zugangsnummer | BC000234 | 
| Gene symbol | NNMT | 
| Gene ID (NCBI) | 4837 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
NNMT can catalyze the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PMID: 21823666; 23455543; 8182091). It plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PMID: 23455543; PMID: 26571212; PMID: 31043742).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for NNMT antibody 15123-1-AP | Protokoll herunterladen | 
| IHC protocol for NNMT antibody 15123-1-AP | Protokoll herunterladenl | 
| IF protocol for NNMT antibody 15123-1-AP | Protokoll herunterladen | 
| IP protocol for NNMT antibody 15123-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Cell Rep Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis | ||
| Oncogene Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids | ||
| Mol Metab Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction. | ||
| Mol Oncol Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.
 | ||
| Front Cell Infect Microbiol Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells. | ||
| Sci Rep The significance of NAD + metabolites and nicotinamide N-methyltransferase in chronic kidney disease. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Kenzo (Verified Customer) (01-05-2024) | This antibody worked for immunofluorescence with a little background 
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| FH Johnny (Verified Customer) (01-07-2022) | products from proteintech have always been reliable. 
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