NNT Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NNT Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 13442-2-AP

Synonyme

NNT

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Galerie der Validierungsdaten

• 

  WB analysis of human adrenal gland using 13442-2-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13442-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13442-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 13442-2-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13442-2-AP

  IP Result of anti-NNT (IP:13442-2-AP, 4ug; Detection:13442-2-AP 1:300) with HepG2 cells lysate 2800ug.

• 

  IHC staining of human liver cancer using 13442-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13442-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13442-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NNT antibody (13442-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13442-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13442-2-AP (NNT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nebennierengewebe, humanes Herzgewebe, humanes Lebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13442-2-AP bindet in WB, IP, IHC, IF, CoIP, ELISA NNT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NNT fusion protein Ag4259
• Vollständiger Name: nicotinamide nucleotide transhydrogenase
• Berechnetes Molekulargewicht: 1085 aa, 114 kDa
• Beobachtetes Molekulargewicht: 114 kDa
• GenBank-Zugangsnummer: BC032370
• Gene symbol: NNT
• Gene ID (NCBI): 23530
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Redox Biol; Nicotinamide nucleotide transhydrogenase-mediated redox homeostasis promotes tumor growth and metastasis in gastric cancer.; Authors - Shuai Li ; KD Validated
rat	WB	Front Cell Dev Biol; Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.; Authors - Hao Zhang
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu
mouse	WB	Cell Physiol Biochem; Effect of Type I Diabetes on the Proteome of Mouse Oocytes.; Authors - Guangjian Jiang
human	WB	Mol Nutr Food Res; Lack of β, β-carotene -9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice.; Authors - Lei Wu
human	WB	Am J Med Genet A; Nicotinamide nucleotide transhydrogenase mutation analysis in Chinese patients with thyroid dysgenesis.; Authors - Miaomiao Li