NOD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NOD2 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 20980-1-AP

Synonyme

Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15, CARD15

---

• 

  IHC staining of human colon using 20980-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20980-1-AP (NOD2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20980-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20980-1-AP (NOD2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 20980-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 20980-1-AP (NOD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

• 

  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

• 

  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

20980-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NOD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nucleotide-binding oligomerization domain containing 2
• Berechnetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: NM_022162
• Gene symbol: NOD2
• Gene ID (NCBI): 64127
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS). The antibody is specific to NOD2.

Publikationen

Species	Application	Title
mouse	WB,IHC	Kidney Int; PMID: 23594678; Authors - Du Pengchao P
mouse	WB	Kidney Int; Progranulin protects against renal ischemia/reperfusion injury in mice.; Authors - Meng Zhou
mouse	WB	Hypertension; Novel Role of NOD2 in Mediating Ca2+ Signaling: Evidence From NOD2-Regulated Podocyte TRPC6 Channels in Hyperhomocysteinemia.; Authors - Han Huirong H
mouse	WB,IF	J Neuroinflammation; NOD2 promotes dopaminergic degeneration regulated by NADPH oxidase 2 in 6-hydroxydopamine model of Parkinson's disease.; Authors - Li Cheng
rat, human	WB, IHC	Free Radic Biol Med; Identification of NOD2 as a novel target of RNA-binding protein HuR:Evidence from NADPH oxidase-mediated HuR signaling in diabetic nephropathy.; Authors - Jin Shang
human	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Effects of peptidoglycan on the development of steatohepatitis.; Authors - Meiling Jin