NOD2 Monoklonaler Antikörper

NOD2 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1A10D1

Kat-Nr. : 66710-1-Ig

Synonyme

ACUG, BLAU, CARD15, CD, CLR16.3, IBD1, NLRC2, NOD2, NOD2B, PSORAS1

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Galerie der Validierungsdaten

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  WB analysis using 66710-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66710-1-Ig (NOD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Raji-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

66710-1-Ig bindet in WB, IF, ELISA NOD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nucleotide-binding oligomerization domain containing 2
• Berechnetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: NM_022162
• Gene symbol: NOD2
• Gene ID (NCBI): 64127
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS).

Publikationen

Species	Application	Title
	WB	Front Pharmacol; CircEpc1 Promotes Ricin Toxin-Induced Inflammation via Activation of NF-κB and MAPK Signaling Pathways by Sponging miR-5114.; Authors - Mingxin Dong
	WB,IF	Front Cell Infect Microbiol; Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways.; Authors - Chun-Xia Shi
	WB	J Cell Mol Med; β-arrestin 2 negatively regulates NOD2 signalling pathway through association with TRAF6 in microglia after cerebral ischaemia/reperfusion injury.; Authors - Lin Chen
human	WB	Front Pharmacol; Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cells; Authors - Yaying You