NOL12 Polyklonaler Antikörper

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• KD/KO Validated

NOL12 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15456-1-PBS

Synonyme

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  WB analysis using 15456-1-AP (same clone as 15456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 15456-1-AP (same clone as 15456-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 15456-1-AP (same clone as 15456-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

  IP result of anti-NOL12 (IP:15456-1-AP, 4ug; Detection:15456-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15456-1-AP (same clone as 15456-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NOL12 antibody (15456-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15456-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA NOL12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOL12 fusion protein Ag7723
• Vollständiger Name: nucleolar protein 12
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC002808
• Gene symbol: NOL12
• Gene ID (NCBI): 79159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleolar protein 12 (NOL12), a multifunctional RNA-binding protein, has been found to be related to genomic stability, DNA damage repair, and apoptosis. High expression of NOL12 is associated with worse reduced overall survival (OS), high pathological grade, node metastasis, and advanced clinical stage in patients with HCC (PMID: 35693698). Knockdown of NOL12 impairs cellular proliferation by inducing a G1/S cell cycle arrest, but does so outside of a nucleolar stress response and in a p53-independent manner (PMID: 29069457).