NOL3 Polyklonaler Antikörper

NOL3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10846-2-PBS

Synonyme

Nop30, NOP, Myp, Muscle-enriched cytoplasmic protein, CARD2

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  WB analysis of mouse skeletal muscle using 10846-2-AP (same clone as 10846-2-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 10846-2-AP (same clone as 10846-2-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10846-2-AP (same clone as 10846-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10846-2-AP (same clone as 10846-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10846-2-AP (NOL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10846-2-AP (same clone as 10846-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10846-2-AP (NOL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10846-2-AP (same clone as 10846-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NOL3 antibody (10846-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10846-2-AP (same clone as 10846-2-PBS)

  Immunofluorescent analysis of HepG2 cells, using NOL3 antibody 10846-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10846-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NOL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOL3 fusion protein Ag1306
• Vollständiger Name: nucleolar protein 3 (apoptosis repressor with CARD domain)
• Berechnetes Molekulargewicht: 24 aa, 3 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC012798
• Gene symbol: NOL3
• Gene ID (NCBI): 8996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.