NOL7 Polyklonaler Antikörper

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• KD/KO Validated

NOL7 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18424-1-AP

Synonyme

C6orf90, NOP27, Nucleolar protein 7, nucleolar protein 7, 27kDa, Nucleolar protein of 27 kDa

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  WB analysis of HEK-293 using 18424-1-AP

  WB result of NOL7 antibody (18424-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOL7 transfected HEK-293 cells.

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  WB analysis using 18424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18424-1-AP (NOL7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18424-1-AP bindet in WB, ELISA NOL7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOL7 fusion protein Ag13272
• Vollständiger Name: nucleolar protein 7, 27kDa
• Berechnetes Molekulargewicht: 257 aa, 29 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC023517
• Gene symbol: NOL7
• Gene ID (NCBI): 51406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOL7 (nucleolar protein 7) is localized in both the nucleolus and nucleoplasm, with its nucleolar localization being RNA-dependent. Its function in the nucleolus is likely related to maintaining nucleolar structure and cell growth, while its role in the nucleoplasm may be associated with anti-angiogenic effects. Additionally, NOL7 is involved in the accumulation of early pre-rRNA and the processing of pre-18S rRNA, which are crucial for maintaining nucleolar function and cell growth. The expression of NOL7 is regulated by various transcription factors, including c-myc and RXRα. In cervical cancer, NOL7 transcription is also positively regulated by the RB tumor suppressor gene. The NOL7 gene encodes three distinct splice variants, each with different intracellular localizations that may affect their functions.